Senior biology students recently had an exciting opportunity to visit the Biochemistry Department at the University of Otago.
The trip provided a hands-on experience with some of the biotechnology techniques that are typically beyond the reach of the school lab.
The lab began with restriction enzymes being used to cut circular DNA molecules, known as plasmids, into fragments at specific base sequences. These enzymes act like molecular scissors, allowing scientists to analyse or modify DNA in precise ways.
Once the DNA was cut, students prepared and ran their samples through a gel electrophoresis. This is a technique that separates DNA fragments based on their size using an electric current. As the current ran through the gel, smaller DNA fragments moved faster and farther than larger ones, creating visible bands when stained and viewed under UV light. This technique is commonly used in research, medicine, and forensic science to identify and compare DNA.
For most students, it was their first time working with micropipettes, restriction enzymes, and gel electrophoresis. It was a fantastic opportunity to gain a deeper understanding of genetic technologies in a real laboratory setting.
We are grateful to Shar Snoeck and her team of students who ran the lab session.
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